A pilot study of radiocesium activity concentration in the stemflow of deciduous broad-leaved trees: Its relationship with leaves and outer bark as of 2022-2023.

The radiocesium (137Cs) activity concentration in stemflow, which is an element of the 137Cs cycle in the forest, is determined by the supply of 137Cs from the outer bark and leaves. Long-term monitoring data of deciduous broad-leaved trees since the Fukushima Daiichi Nuclear Power Plant (FDNPP) accident have shown the 137Cs activity concentration in the outer bark decreasing exponentially, while that in the leaves has remained nearly constant. Therefore, it is expected that the contribution of the outer bark to 137Cs in stemflow eventually becomes smaller than that of leaves over time. We hypothesized that as of 2022-2023 (i.e., more than 10 years after the FDNPP accident), the main source of 137Cs in the stemflow of deciduous broad-leaved trees is mainly leaching from leaves. We tested this hypothesis by conducting two surveys in the autumn of 2022 (September-October) and early summer of 2023 (May-June) in Kawauchi Village, Fukushima Prefecture. Samples consisted of stemflow, leaves, and outer bark from a total of 10 deciduous broad-leaved trees (three konara oaks, three mizunara oaks, two Japanese chestnuts, and two cherry blossoms). Our statistical analyses (correlation, linear and multilinear regression analyses) showed that the 137Cs activity concentrations in stemflow were significantly positively correlated to those in leaves, with no positive correlation detected with the outer bark, suggesting that at the time of the survey, the 137Cs activity concentration in stemflow was mainly influenced by the 137Cs activity concentration in leaves. In addition, we propose a method for estimating the stemflow 137Cs activity concentration in konara oak using data from leaves. Although the method's prediction accuracy is low from 2011 to 2013, it is able to estimate the stemflow 137Cs activity concentration in konara oak. Thus, it can help determine one of the model parameters of 137Cs dynamics within deciduous broad-leaved forests.

Effect of litter removal five years after the Fukushima accident on 137Cs uptake by Japanese cedar.

Removal of litter-associated 137Cs from the forest floor (litter removal) can reduce the 137Cs uptake by plants; however, the proposed effective period for litter removal was 1-2 years after the Fukushima Dai-ichi Nuclear Power Plant (FDNPP) accident. This is because the 137Cs in forest soil migrates rapidly from the litter to surface mineral soil layers in Japanese forests, and thus the effectiveness of litter removal will quickly become limited. However, it is unknown whether this approach can be applied to forests whose vertical migration of 137Cs in the forest soil is relatively slow. Herein, we compared the 137Cs activity concentration in the inner bark of the Japanese cedar (Cryptomeria japonica) between litter removal (conducted in September and October 2016, 5 years after the accident) and in control areas in Kawauchi Village, Fukushima Prefecture, where the vertical migration of 137Cs was relatively slow from the litter to surface mineral soil layers. Air dose rates (ambient dose equivalent) in the litter removal area were significantly lower than those in the control area in 2022, and the 137Cs inventory in the forest soil in litter removal area also tended to be lower than that in the control area. In Japanese cedars with similar levels of outer bark contamination, the 137Cs activity concentration in the inner bark in the litter removal area was significantly lower than that in the control area, and consistent trends were also found when comparing the 137Cs activity concentration in the leaves of Stephanandra incisa and Wisteria floribunda obtained from the same forest. Thus, the litter removal 5 years after the FDNPP accident may have reduced the 137Cs uptake in Japanese cedar in an evergreen coniferous forest where the vertical migration of 137Cs is relatively slow in the forest soil.

Radiocesium mobility in different parts of the two major tree species in Fukushima.

Radiocesium (137Cs) released in the Fukushima Dai-ichi Nuclear Power Plant accident is still cycling in the forest ecosystem. We examined the mobility of 137Cs in the external parts-leaves/needles, branches, and bark-of the two major tree species in Fukushima, Japanese cedar (Cryptomeria japonica) and konara oak (Quercus serrata). This variable mobility will likely lead to spatial heterogeneity of 137Cs and difficulty in predicting its dynamics for decades. We conducted leaching experiments on these samples by using ultrapure water and ammonium acetate. In Japanese cedar, the 137Cs percentage leached from current-year needles was 26-45% (ultrapure water) and 27-60% (ammonium acetate)-similar to those from old needles and branches. In konara oak, the 137Cs percentage leached from leaves was 47-72% (ultrapure water) and 70-100% (ammonium acetate)-comparable to those from current-year and old branches. Relatively poor 137Cs mobility was observed in the outer bark of Japanese cedar and in organic layer samples from both species. Comparison of the results from corresponding parts revealed greater 137Cs mobility in konara oak than in Japanese cedar. We suggest that more active cycling of 137Cs occurs in konara oak.

Deciphering nitrous oxide emissions from tropical soils of different land uses.

Tropical regions are hotspots of increasing greenhouse gas emissions associated with land-use change. Although many field studies have quantified soil fluxes of nitrous oxide (N2O; a potent greenhouse gas) from various land uses, the driving mechanisms remain uncertain. Here, we used tropical soils of diverse land uses and actively manipulated the soil moisture (35%, 60%, and 95% water-filled pore space [WFPS]) and substrate supply (control, nitrate, and nitrate plus glucose) to investigate the responses of N2O emissions with short-term incubations. We then identified key factors regulating N2O emissions out of a series of soil physicochemical and biological factors and explored how these factors interacted to drive N2O emissions. Land-use changes from primary forest to oil palm or Acacia plantation risks emitting more N2O, whereas low emissions could be maintained by conversion to Macaranga forest or Imperata grassland; these laboratory observations were corroborated by a literature synthesis of field N2O measurements across tropical regions. Soil redox potential (Eh) and labile organic nitrogen (LON; amino acid mixture, arginine, and urea) mineralization were among the factors with greatest influence on N2O emissions. In contrast to common understandings, the control of WFPS over N2O emissions was largely indirect, and acted through Eh. The mineralization of LON, particularly arginine, potentially played multiple roles in N2O production (e.g., bottlenecks of nitrifier-denitrification or simultaneous nitrification-denitrification versus substrate competition for co-denitrification). Structural equation models suggest that soil-environmental factors of different levels (from distal including land use, soil moisture, and pH to proximal such as LON mineralization) drive N2O emissions through cascading interactions. Overall, we show that, despite identical initial soil conditions, land conversion can substantially alter the N2O emission potential. Also, collectively considering soil-environmental regulators and their interactions associated with land conversion is crucial to predict and design mitigation strategies for N2O emissions from land-use change.

Ten-year trends in vertical distribution of radiocesium in Fukushima forest soils, Japan.

To elucidate interannual changes in the vertical distribution of 137Cs in forest ecosystems contaminated by the Fukushima Dai-ichi Nuclear Power Plant accident, we investigated 137Cs inventories in forest soils (both organic and mineral soil horizons) at 10 sampling plots with different 137Cs deposition levels and dominant species for up to 10 years after the accident. We examined the temporal variation of the 137Cs inventories by depth with exponential regression models (assuming that the transition and partitioning of 137Cs are still active) and exponential offset regression models (assuming a shift to a stable 137Cs distribution, defined as the "quasi-equilibrium steady-state" in the Chernobyl accident). In the organic horizon, the 137Cs inventories were exponentially decreasing, and it might take more time to converge in the quasi-equilibrium steady-state at most plots. In the mineral soil horizon, most of 137Cs was found in the surface layer of the mineral soil horizon (0-5 cm). In this layer, the inventories first increased and then become relatively constant, and the exponential offset model was selected at most plots, suggesting entry into the quasi-equilibrium steady-state over the observation period. Although we also observed exponentially increasing trends in a lower layer (5-10 cm) of the mineral soil horizon, there was no clear increasing or decreasing trend of 137Cs inventory in the deeper mineral soil layers (10-15 and 15-20 cm). Our calculation of the relaxation depth and migration center revealed that downward migration of 137Cs is not significant in terms of the overall 137Cs distribution in the mineral soil horizon over 10 years.

Intraoperative Cerebral Measurements Using Implantable Cortical Multimodality Probe.

In this study, a multimodality probe that simultaneously measures electroencephalograms, cerebral hemodynamics, and brain surface temperature was developed. This probe has six channels, and each channel has a platinum electrode for cortical electroencephalogram measurements, light emitting diodes, and photodiodes for hemodynamic measurements using near-infrared spectroscopy (NIRS), and a thermistor for measuring the cerebral surface temperature (BrT). A probe with a width of 8.0 mm and maximum total thickness of 0.7 mm was fabricated using flexible printed circuit board technology for chronic intracranial placement. Brain activity using the prototype probe at the resected site was measured and its function performance was evaluated. Characteristic epileptogenic abnormal electroencephalograms accompanied by polarity reversal between channels occurred at 16 min and 38 s. It was concluded that the brain cells consumed oxygen during the occurrence of abnormal electroencephalograms. At this time, no noticeable change in HbT values could be confirmed.

Reduction of gibberellin by low temperature disrupts pollen development in rice.

Microsporogenesis in rice (Oryza sativa) plants is susceptible to moderate low temperature (LT; approximately 19°C) that disrupts pollen development and causes severe reductions in grain yields. Although considerable research has been invested in the study of cool-temperature injury, a full understanding of the molecular mechanism has not been achieved. Here, we show that endogenous levels of the bioactive gibberellins (GAs) GA4 and GA7, and expression levels of the GA biosynthesis genes GA20ox3 and GA3ox1, decrease in the developing anthers by exposure to LT. By contrast, the levels of precursor GA12 were higher in response to LT. In addition, the expression of the dehydration-responsive element-binding protein DREB2B and SLENDER RICE1 (SLR1)/DELLA was up-regulated in response to LT. Mutants involved in GA biosynthetic and response pathways were hypersensitive to LT stress, including the semidwarf mutants sd1 and d35, the gain-of-function mutant slr1-d, and gibberellin insensitive dwarf1. The reduction in the number of sporogenous cells and the abnormal enlargement of tapetal cells occurred most severely in the GA-insensitive mutant. Application of exogenous GA significantly reversed the male sterility caused by LT, and simultaneous application of exogenous GA with sucrose substantially improved the extent of normal pollen development. Modern rice varieties carrying the sd1 mutation are widely cultivated, and the sd1 mutation is considered one of the greatest achievements of the Green Revolution. The protective strategy achieved by our work may help sustain steady yields of rice under global climate change.

Community structures of Mesostigmata, Prostigmata and Oribatida in broad-leaved regeneration forests and conifer plantations of various ages.

The community structures of Mesostigmata, Prostigmata, and Oribatida in the soil of broad-leaved regeneration forests and conifer plantations of various ages were assessed alongside soil and plant environmental variables using three response metrics (density, species richness, and species-abundance distribution). The density and species richness of mites recovered swiftly after clear-cutting or replanting. Oribatid mites dominated the soil mite communities in terms of densities and species richness for both forest types. Soil mite communities in broad-leaved forests was related to forest age, the crown tree communities index, and forest-floor litter weight. In contrast, soil mite communities in the conifer plantation sites were related to various indices of understory plants. The development of the understory plants was synchronized with the silvicultural schedules, including a closed canopy and thinning. Such a conifer plantation management may affect indirectly the community of mites.

Increasing trends of soil greenhouse gas fluxes in Japanese forests from 1980 to 2009.

Forest soils are a source/sink of greenhouse gases, and have significant impacts on the budget of these terrestrial greenhouse gases. Here, we show climate-driven changes in soil GHG fluxes (CO2 emission, CH4 uptake, and N2O emission) in Japanese forests from 1980 to 2009, which were estimated using a regional soil GHG model that is data-oriented. Our study reveals that the soil GHG fluxes in Japanese forests have been increasing over the past 30 years at the rate of 0.31 Tg C yr⁻² for CO2 (0.23 % yr⁻¹, relative to the average from 1980 to 2009), 0.40 Gg C yr⁻² for CH4 (0.44 % yr⁻¹), and 0.0052 Gg N yr⁻² for N2O (0.27 % yr⁻¹). Our estimates also show large interannual variations in soil GHG fluxes. The increasing trends and large interannual variations in soil GHG fluxes seem to substantially affect Japan's Kyoto accounting and future GHG mitigation strategies.

Tissue-specific auxin signaling in response to temperature fluctuation.

Auxin levels are well regulated in cells and tissues by both transport and local biosynthesis, and its distribution is important for the modulation of cell proliferation, differentiation, development, tropisms and high-temperature response. Activation of auxin biosynthesis with increased temperatures reported in certain plant tissues. In contrast, our studies indicated that male tissue-specific auxin reduction via transcriptional repression of the YUCCA auxin biosynthesis genes is the primary cause of high temperature injury, which leads the abortion of pollen development in Arabidopsis and barley Hordeum vulgare L. Furthermore, the abortion can be reversed by the application of exogenous auxin, suggesting that the application may maintain crop yields during the current global warming crisis.

Inhibitory effect of yogurt on aberrant crypt foci formation in the rat colon and colorectal tumorigenesis in RasH2 mice.

The inhibitory effects of yogurt consisting of milk fermented by Lactobacillus delbrueckii subsp. bulgaricus strain 2038 and Streptococcus salivarius subsp. thermophilus strain 1131 on formation of colonic aberrant crypt foci (ACF) in rats and also on development of colorectal tumors in transgenic mice harboring human prototype c-Ha-ras genes (rasH2 mice) were examined. F344 rats and rasH2 mice were fed commercial diet containing freeze-dried yogurt or starter medium (non-fermented milk). Rats were inoculated orally with heterocyclic amine 2-amino-methyl-6-phenylimidazo[4,5-b]pyridine hydrochloride (PhIP) for two weeks. The rats were necropsied 14 days after the PhIP treatment, and ACF in the colon and rectum were counted. RasH2 mice were injected with 1,2-dimethylhydrazine dihydrochloride (DMH) for 20 weeks. Three weeks after the last injection of DMH, rasH2 mice were necropsied to determine the number and the size of colorectal tumors. Yogurt supplementation in diet significantly reduced the number of ACF and aberrant crypts (ACs) in rats fed control diet (P<0.01), but not in rats fed non-fermented milk diet. On the other hand, rasH2 mice receiving the yogurt-supplemented diet had significantly reduced numbers of tumors induced by DMH compared with those fed the non-fermented milk-supplemented diet (P<0.05). These results demonstrate that the yogurt used in this study appears to have tumor-suppressing properties, and rasH2 mice are a useful model for the evaluation of antitumor activities of foods.

Auxins reverse plant male sterility caused by high temperatures.

With global warming, plant high temperature injury is becoming an increasingly serious problem. In wheat, barley, and various other commercially important crops, the early phase of anther development is especially susceptible to high temperatures. Activation of auxin biosynthesis with increased temperatures has been reported in certain plant tissues. In contrast, we here found that under high temperature conditions, endogenous auxin levels specifically decreased in the developing anthers of barley and Arabidopsis. In addition, expression of the YUCCA auxin biosynthesis genes was repressed by increasing temperatures. Application of auxin completely reversed male sterility in both plant species. These findings suggest that tissue-specific auxin reduction is the primary cause of high temperature injury, which leads to the abortion of pollen development. Thus, the application of auxin may help sustain steady yields of crops despite future climate change.

Retinoblastoma and its binding partner MSI1 control imprinting in Arabidopsis.

Parental genomic imprinting causes preferential expression of one of the two parental alleles. In mammals, differential sex-dependent deposition of silencing DNA methylation marks during gametogenesis initiates a new cycle of imprinting. Parental genomic imprinting has been detected in plants and relies on DNA methylation by the methyltransferase MET1. However, in contrast to mammals, plant imprints are created by differential removal of silencing marks during gametogenesis. In Arabidopsis, DNA demethylation is mediated by the DNA glycosylase DEMETER (DME) causing activation of imprinted genes at the end of female gametogenesis. On the basis of genetic interactions, we show that in addition to DME, the plant homologs of the human Retinoblastoma (Rb) and its binding partner RbAp48 are required for the activation of the imprinted genes FIS2 and FWA. This Rb-dependent activation is mediated by direct transcriptional repression of MET1 during female gametogenesis. We have thus identified a new mechanism required for imprinting establishment, outlining a new role for the Retinoblastoma pathway, which may be conserved in mammals.

Drosophila Nedd4 regulates endocytosis of notch and suppresses its ligand-independent activation.

BACKGROUND: Ligand-induced proteolytic cleavage and internalization of the plasma membrane receptor Notch leads to its activation. Ligand-independent, steady-state internalization of Notch, however, does not lead to activation. The mechanism by which downstream effectors discriminate between these bipartite modes of Notch internalization is not understood. Nedd4 is a HECT domain-containing E3 ubiquitin ligase that targets transmembrane receptors containing the PPSY motif for endocytosis. Deltex is a positive Notch signaling regulator that encodes a putative ubiquitin ligase of the ring finger type. RESULTS: We used the Drosophila system to show that Notch is ubiquitinated and destabilized by Nedd4 in a manner requiring the PPSY motif in the Notch intracellular domain. Loss of Nedd4 function dominantly suppresses the Notch and Deltex mutant phenotypes, and its hyperactivation attenuates Notch activity. In tissue culture cells, the dominant-negative form of Nedd4 blocks steady-state Notch internalization and activates Notch signaling independently of ligand binding. This effect was further potentiated by Deltex. Nedd4 destines Deltex for degradation in a Notch-dependent manner. CONCLUSIONS: Nedd4 antagonizes Notch signaling by promoting degradation of Notch and Deltex. This Nedd4 function may be important for protecting unstimulated cells from sporadic activation of Notch signaling.